2Z8O
Structural basis for the catalytic mechanism of phosphothreonine lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 70.755, 70.755, 200.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.215 |
| Number of reflections | 20394 | |
| <I/σ(I)> | 63.8 | 17.3 |
| Completeness [%] | 98.1 | 90.9 |
| Redundancy | 41.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.8M Na, K-tartrate, 100mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
