2Z8L
Crystal Structure of the Staphylococcal superantigen-like protein SSL5 at pH 4.6 complexed with sialyl Lewis X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.625, 78.237, 55.276 |
| Unit cell angles | 90.00, 127.02, 90.00 |
Refinement procedure
| Resolution | 28.050 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m4v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.744 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.667 |
| Number of reflections | 30702 | |
| <I/σ(I)> | 25.6 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 20% PEG 3350, 0.2M NaH2PO4, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
