2Z8D

The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-biose

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	106.326, 143.551, 114.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 1.850
R-factor	0.18767
Rwork	0.185
R-free	0.23785
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.518
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.047	0.264
Number of reflections	74117
<I/σ(I)>	42.2	5.2
Completeness [%]	99.0	98.3
Redundancy	7.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	22% PEG MME 550, 0.1M 2-Morpholinoethanesulfonic acid monohydrate, 0.01M zinc sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	22% PEG MME 550, 0.1M 2-Morpholinoethanesulfonic acid monohydrate, 0.01M zinc sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K