2Z7K
tRNA-Guanine transglycosylase (TGT) in complex with 2-Amino-lin-Benzoguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97803 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.500, 65.100, 70.600 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.280 |
| Rwork | 0.162 |
| R-free | 0.18580 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1p0d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 102059 | |
| <I/σ(I)> | 21.4 | 3.8 |
| Completeness [%] | 97.4 | 99.6 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 100mM TRIS HCl, 1mM DTT, 10% DMSO, 5% PEG 8000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
