2Z7E

Crystal structure of Aquifex aeolicus IscU with bound [2Fe-2S] cluster

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.7407, 1.7423, 1.6947, 1.5000
Spacegroup name	P 21 21 2
Unit cell lengths	72.903, 122.136, 62.051
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.250 - 2.300
R-factor	0.225
Rwork	0.225
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.250
High resolution limit [Å]	2.300
Number of reflections	24440
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6M ammonium sulfate, 0.5M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K