2Z74
T. tengcongensis glmS ribozyme bound to glucose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 181.110, 40.060, 70.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
Rwork | 0.209 |
R-free | 0.23500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.004 |
RMSD bond angle | 1.529 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 24645 | |
<I/σ(I)> | 25.2 | 2.6 |
Completeness [%] | 90.8 | 67.9 |
Redundancy | 4.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | GROWN FROM: 12% PEG 4000, 1M LICL, 100MM MES-NAOH PH 5.4, 5MM MGCL2 STABILIZED IN: 24% PEG 4000, 1.5M LICL, 30MM MGCL2, 100MM MES-NAOH PH 5.5, 20MM GLUCOSE-6-PHOSPHATE, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
10 | 1 | 2 | HOH | ||
11 | 1 | 3 | PEG 4000 | ||
12 | 1 | 3 | LiCl | ||
13 | 1 | 3 | MES-NaOH | ||
14 | 1 | 3 | MgCl2 | ||
15 | 1 | 3 | glucose-6-phosphate | ||
2 | 1 | 1 | LiCl | ||
3 | 1 | 1 | MES-NaOH | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG 4000 | ||
7 | 1 | 2 | LiCl | ||
8 | 1 | 2 | MES-NaOH | ||
9 | 1 | 2 | MgCl2 |