2Z6V
Crystal structure of sulfotransferase STF9 from Mycobacterium avium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 229.517, 229.517, 56.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.600 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.098 | 0.427 |
| Number of reflections | 23316 | |
| <I/σ(I)> | 25.9 | 3.6 |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 14.2 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.4 | 1.6M Magnesium sulfate, 0.1M MES (pH 6.5), 0.15M Sodium choloride, 10mM PAP, 10mM DTT, 50mM Tris-HCl (pH 7.0) , VAPOR DIFFUSION, HANGING DROP, temperature 293.4K |
