2Z6B
Crystal Structure Analysis of (gp27-gp5)3 conjugated with Fe(III) protoporphyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Detector technology | CCD |
| Collection date | 2006-12-08 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 138.536, 138.536, 389.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.640 - 3.110 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wth |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 26124 | |
| <I/σ(I)> | 37.8 | 6.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.9 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | PEG 8000, Tris, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
