2Z5J
Free Transportin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.467, 107.467, 194.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.360 - 3.400 |
| R-factor | 0.2358 |
| Rwork | 0.232 |
| R-free | 0.30301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qbk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.085 | 0.374 |
| Number of reflections | 14806 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 94.8 | 72.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 300 | 6% PEG 8000, 0.1M sodium dihidrogen phosphate, 0.1M CAPSO pH 9.8, 0.04M spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
