2Z5C
Crystal Structure of a Novel Chaperone Complex for Yeast 20S Proteasome Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-08 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 158.687, 159.181, 65.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.380 - 2.900 |
| R-factor | 0.2513 |
| Rwork | 0.250 |
| R-free | 0.28334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Z5B; PDB ENTRY 1RYP chain E |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.757 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.380 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 37091 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 8%(v/v) ethyleneglycol, 12%(w/v) PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
