2Z5B

Crystal Structure of a Novel Chaperone Complex for Yeast 20S Proteasome Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-18
Detector	Bruker DIP-6040
Wavelength(s)	0.9000
Spacegroup name	P 31
Unit cell lengths	57.487, 57.487, 82.206
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.810 - 1.960
R-factor	0.24185
Rwork	0.240
R-free	0.28727
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.393
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.810	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.059	0.187
Number of reflections	21419
<I/σ(I)>	24.6
Completeness [%]	98.0	93.7
Redundancy	4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25mM MES, 50mM KH2PO4, 16%(w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25mM MES, 50mM KH2PO4, 16%(w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K