2Z4J

Crystal structure of AR LBD with SHP peptide NR Box 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	CCD
Collection date	2004-09-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.11589
Spacegroup name	P 21 21 21
Unit cell lengths	55.979, 66.542, 71.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.410 - 2.600
R-factor	0.207
Rwork	0.207
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t63
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.410
High resolution limit [Å]	2.600	2.600
Number of reflections	7836
Completeness [%]	90.3	90.5
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M Bis-Tris Propane, 1.2M Potassium Sodium Tartrate tetrahydrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K