2Z4H

Crystal structure of the Cpx pathway activator NlpE from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	95
Detector technology	CCD
Collection date	2004-11-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1.6000
Spacegroup name	P 43 21 2
Unit cell lengths	121.155, 121.155, 83.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.310 - 2.800
Rwork	0.241
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	15130
<I/σ(I)>	22.6	2.4
Completeness [%]	95.0	75.3
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	14% PEG 8000, 0.05M sodium citrate, 0.16M zinc sulfate, 40% D-sorbitol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4	298	14% PEG 8000, 0.05M sodium citrate, 0.16M zinc sulfate, 40% D-sorbitol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K