2Z47
The Y66L mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.424, 69.558, 53.749 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 30.830 - 1.600 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 4.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.830 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 33331 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 70% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
