2Z42
Crystal Structure of Family 7 Alginate Lyase A1-II' from Sphingomonas sp. A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.335, 41.329, 133.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.670 - 1.600 |
| R-factor | 0.17863 |
| Rwork | 0.177 |
| R-free | 0.21754 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cws |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.670 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.197 |
| Number of reflections | 30750 | |
| <I/σ(I)> | 35.4 | 5.7 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 2.0M ammonium sulfate, 5% PEG 400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 293K |
