2Z3G

Crystal structure of blasticidin S deaminase (BSD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	54.116, 68.762, 146.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 1.500
R-factor	0.175
Rwork	0.174
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wn5
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Rmerge	0.078
Number of reflections	88094
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG8000, 50MM TRIS, 50MM MAGNESIUM CHLORIDE, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K