2Z3F
Crystal structure of spCia1/Asf1 complexed with Cac2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 151.512, 151.512, 144.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.600 - 2.700 |
| R-factor | 0.21103 |
| Rwork | 0.208 |
| R-free | 0.26319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cu9 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.749 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.480 |
| Number of reflections | 50092 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.8M Na/K phosphate pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
