2Z36
Crystal structure of cytochrome P450 MoxA from Nonomuraea recticatena (CYP105)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.557, 83.256, 175.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.600 - 2.800 |
| Rwork | 0.230 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jin |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.249 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 29919 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 8% ethyleneglycol, 4% PEG 8000, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
