2Z2U
Crystal structure of archaeal TYW1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.179, 68.659, 75.252 |
| Unit cell angles | 90.00, 95.75, 90.00 |
Refinement procedure
| Resolution | 45.130 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 11855 | |
| <I/σ(I)> | 43.7 | 18.8 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2M sodium potassium tartrate, 20%(w/v) PEG3350, 3%(v/v) PEG400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
