2Z26
Thr110Ala dihydroorotase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.493, 78.885, 180.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.290 |
| R-factor | 0.131 |
| Rwork | 0.129 |
| R-free | 0.15400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xge |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.320 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.071 | 0.363 |
| Number of reflections | 166743 | |
| <I/σ(I)> | 20.5 | 3 |
| Completeness [%] | 91.1 | 49.8 |
| Redundancy | 6.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277 | 17% PEG3350, 0.1M MES, 75mM MgCl2, 0.15M KCl, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
