2Z1S
Beta-glucosidase B from paenibacillus polymyxa complexed with cellotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.324, 75.283, 88.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.080 - 2.460 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.242 | 2.580 |
| High resolution limit [Å] | 2.457 | 2.450 |
| Rmerge | 0.121 | 0.580 |
| Total number of observations | 9628 | |
| Number of reflections | 17936 | |
| <I/σ(I)> | 5 | 1.3 |
| Completeness [%] | 98.3 | 89.2 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG550MME, 0.05M CL2CA, 0.1M BIS/TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
