2Z1O
Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.492, 103.516, 122.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.750 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.368 |
| Number of reflections | 94532 | |
| <I/σ(I)> | 5.87 | |
| Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 90% of 0.1M HEPES (pH = 7.5) solution with 20% PEG 10000, 8% ethylene glycol, 10% of 0.1 M Tris/HCl (pH = 8.5) solution, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
