2Z1E
Crystal structure of HypE from Thermococcus kodakaraensis (outward form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.345, 45.833, 75.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.070 - 1.550 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 44379 | |
| <I/σ(I)> | 34.5 | 4.2 |
| Completeness [%] | 98.5 | 89.2 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M acetic acid, 22%(w/v) MPD, 200mM NaCl, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
