2Z12
Structure of the transformed monoclinic lysozyme by controlled dehydration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-01-05 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.947, 58.021, 30.580 |
| Unit cell angles | 90.00, 110.40, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.150 |
| Rwork | 0.168 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d4j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.061 | 0.112 |
| Number of reflections | 29957 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.75 | 2.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Dehydration induced phase transition | 293 | temperature-regulated nitragen gas, Dehydration induced phase transition, temperature 293K |
