2Z10

Crystal structure of putative acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-07-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	71.401, 71.401, 99.255
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.920 - 1.770
Rwork	0.206
R-free	0.23700
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	28933
<I/σ(I)>	34.3	8.9
Completeness [%]	98.9	90.8
Redundancy	9.7	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.15M ammonium acetate, 0.1M bis-tris pH5.5, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K