2Z0U

Crystal structure of C2 domain of KIBRA protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-05
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790
Spacegroup name	P 21 21 2
Unit cell lengths	88.456, 88.928, 36.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 2.200
R-factor	0.22
Rwork	0.220
R-free	0.27400
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.460	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	15280
<I/σ(I)>	22.6
Completeness [%]	99.8	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293K