2Z0S

Crystal structure of putative exosome complex RNA-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00
Spacegroup name	P 61 2 2
Unit cell lengths	97.414, 97.414, 136.548
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.710 - 3.200
R-factor	0.277
Rwork	0.277
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ba0
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	6813
<I/σ(I)>	43.6
Completeness [%]	100.0	100
Redundancy	19.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% Ethanol, 1.5M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K