2Z0R
Crystal structure of hypothetical protein TTHA0547
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97906, 0.97943, 0.964 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.501, 94.297, 101.734 |
Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
Resolution | 42.840 - 2.300 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.840 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 56109 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.7 | 97.6 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.45M Ammonium sulfate, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |