2Z0Q

Crystal structure of DH-PH domain of RhoGEF3(Xpln)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-31
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97888, 0.97964, 0.9640
Spacegroup name	P 21 21 21
Unit cell lengths	51.582, 62.670, 115.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.430 - 1.790
R-factor	0.203
Rwork	0.203
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.790	1.790
Number of reflections	35822
<I/σ(I)>	28.2
Completeness [%]	99.4	96.4
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEGMME5000, Ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K