2Z0O

Crystal structure of APPL1-BAR-PH domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9792, 0.964, 0.97942
Spacegroup name	P 21 21 2
Unit cell lengths	103.449, 111.003, 36.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.700 - 2.580
R-factor	0.235
Rwork	0.235
R-free	0.29900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	2.670
High resolution limit [Å]	2.580	2.580
Number of reflections	13536
<I/σ(I)>	14.1
Completeness [%]	98.1	99
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% PEG3350, 0.2M Ammonium acetate, 0.1M Bis-tris, 0.3M Non-Detergent Sulfo-betaine 195, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K