2Z0J
Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9955, 0.9814, 0.9717, 0.9920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.860, 65.004, 176.266 |
| Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
| Resolution | 45.940 - 1.500 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 320561 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1M MES pH 6.8, 12% PEG 8000, 20% glycerol, 0.16M CaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
