2Z0B
Crystal structure of CBM20 domain of human putative glycerophosphodiester phosphodiesterase 5 (KIAA1434)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-22 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.979270, 0.979740, 0.96400 |
| Spacegroup name | P 31 |
| Unit cell lengths | 111.040, 111.040, 77.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 65368 | |
| <I/σ(I)> | 29.9 | 3.8 |
| Completeness [%] | 90.7 | 50.4 |
| Redundancy | 5.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.0M Sodium/Potassium Phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
