2Z08
Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 33.100, 75.050, 88.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.550 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wjg |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.035 | 0.110 |
| Number of reflections | 17645 | |
| <I/σ(I)> | 40.9 | 6.85 |
| Completeness [%] | 97.2 | 75.1 |
| Redundancy | 6.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 11mM ATP, 40mM Magnesium Chloride, 0.1M Sodium acetate trihydrate, 2.0M Sodium chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
