2YZY

Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-28
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	41.380, 58.840, 80.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.229
Rwork	0.229
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.032	0.089
Number of reflections	26453
<I/σ(I)>	44.1	17.7
Completeness [%]	99.2	95.8
Redundancy	6.8	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M Sodium acetate, 15% PEG 4000, 29% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M Sodium acetate, 15% PEG 4000, 29% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M Sodium acetate, 15% PEG 4000, 29% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K