2YZT

Crystal structure of uncharacterized conserved protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-18
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9000, 0.9794
Spacegroup name	P 31 2 1
Unit cell lengths	50.152, 50.152, 46.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.430 - 1.800
R-factor	0.242
Rwork	0.242
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.197
Number of reflections	6606
<I/σ(I)>	39.6	9.2
Completeness [%]	99.9	99.4
Redundancy	9.9	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.2M di-Ammonium H citrate, 0.1M Sodium citrate pH5.0, 15% PEG3350, 10MM Phenol, VAPOR DIFFUSION, HANGING DROP, temperature 293K