2YZQ

Crystal structure of uncharacterized conserved protein from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-05
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	98.753, 75.008, 49.661
Unit cell angles	90.00, 119.53, 90.00

Refinement procedure

Resolution	29.960 - 1.630
R-factor	0.225
Rwork	0.225
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.630	1.630
Rmerge	0.060	0.153
Number of reflections	73474
Completeness [%]	95.4	84.9
Redundancy	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2M Lithium Sulfate, 30% PEG400, 0.1M Tris-HCl, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2M Lithium Sulfate, 30% PEG400, 0.1M Tris-HCl, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K