2YZO

Crystal structure of uncharacterized conserved protein from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	65.791, 71.732, 181.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.221
Rwork	0.221
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	37099
Completeness [%]	99.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	0.1M MES pH6.6, 10% iso-Propanol, 35% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K