2YZH
Crystal structure of peroxiredoxin-like protein from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.118, 81.642, 70.909 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 47.210 - 1.850 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1psq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.099 | 0.276 |
| Number of reflections | 56115 | |
| <I/σ(I)> | 19.48 | 3.46 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 5.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2M Magnesium sulfate, 0.1M tri-Sodium citrate pH4.2, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
