2YZ2
Crystal structure of the ABC transporter in the cobalt transport system
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97891, 0.90000, 0.97934 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 148.499, 148.499, 106.967 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.21751 |
| Rwork | 0.214 |
| R-free | 0.29403 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.999 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.117 | 0.490 |
| Number of reflections | 31555 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.3 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M potassium flouride, 20% (w/v) PEG 3350 (PEG/ION-2), pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
