2YYT

Crystal structure of uncharacterized conserved protein from Geobacillus kaustophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-07
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	47.213, 58.860, 88.135
Unit cell angles	100.74, 96.56, 107.44

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.229
Rwork	0.229
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	141451
Completeness [%]	100.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Sodium Sulfate decahydrate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K