2YYH
Crystal structure of Nudix family protein from Aquifex aeolicus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97894, 0.9000, 0.97935 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.020, 34.336, 130.005 |
Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
Resolution | 39.500 - 1.800 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 91555 | |
<I/σ(I)> | 18 | 1.96 |
Completeness [%] | 98.5 | 91 |
Redundancy | 2.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 0.16M LiCl, 16% PEG3350, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |