2YY9

Crystal structure of BTB domain from mouse HKR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-06
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	67.821, 67.821, 216.487
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.550 - 2.600
R-factor	0.226
Rwork	0.226
R-free	0.28900
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.600	2.600
Number of reflections	9810
<I/σ(I)>	39.1	10.1
Completeness [%]	100.0
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293	0.056M sodium dihydrogen phosphate, 1.344M di-potassium hydrogen phosphate, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K