2YY6
Crystal Structure of the phosphoglycolate phosphatase from Aquifex aeolicus VF5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 31 |
| Unit cell lengths | 96.247, 96.247, 50.703 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nyv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.294 |
| Number of reflections | 23364 | |
| <I/σ(I)> | 5.3 | 5.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 10% PEG6000, 0.5M Li-Sulfate, 0.1M Na-Acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
