2YXX
Crystal structure analysis of Diaminopimelate decarboxylate (lysA)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97898, 0.90000, 0.97946 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.818, 98.276, 109.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.078 | 0.360 |
| Number of reflections | 60672 | |
| Completeness [%] | 98.7 | 88.6 |
| Redundancy | 6.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil-batch | 4.5 | 293 | 40%(v/v) PEG600, 0.1M Acetate, 0.2M MgCl2, pH 4.5, oil-batch, temperature 293K |
