2YXS

Crystal Structure of N-terminal domain of human galectin-8 with D-lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-22
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.913, 50.551, 69.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.990 - 2.130
R-factor	0.19
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yv8
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.130	2.130
Number of reflections	9421
<I/σ(I)>	32.8	12.6
Completeness [%]	99.7	99.2
Redundancy	6.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M bis-tris, 25% PEG3350, 1mM lactose, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K