2YXH
Crystal structure of mazG-related protein from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97905, 0.90000, 0.97945 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.829, 56.788, 61.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21472 |
| Rwork | 0.212 |
| R-free | 0.26312 |
| Structure solution method | MAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.258 |
| Number of reflections | 18408 | |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 25% PEG3350, 0.2M MgAcetate, 0.1M Tris-HCl, pH8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
