2YXE
Crystal structure of L-isoaspartyl protein carboxyl methyltranferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.267, 84.448, 123.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jg4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.037 | |
| Number of reflections | 42769 | |
| Completeness [%] | 98.1 | 84.5 |
| Redundancy | 6.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil batch | 4.5 | 298 | 0.1M Acetate, 40% 1,2-propanediol, pH 4.5, Oil batch, temperature 298.0K |
