2YXD
Crystal Structure of Cobalamin biosynthesis precorrin 8W decarboxylase (cbiT)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97891, 0.90000, 0.97928 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.442, 93.442, 81.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.20171 |
| Rwork | 0.198 |
| R-free | 0.27322 |
| Structure solution method | MAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.360 |
| Number of reflections | 16322 | |
| Completeness [%] | 98.9 | 94.4 |
| Redundancy | 12.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | OIL BATCH | 6 | 298 | 50% PEG 400, 0.1M MES, pH 6.0, OIL BATCH, temperature 298.0K |
