2YXC

The H25M mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-26
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.942, 67.156, 34.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.230 - 1.500
R-factor	0.178
Rwork	0.178
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j0o
RMSD bond length	0.015
RMSD bond angle	4.500
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	12.230
High resolution limit [Å]	1.500
Number of reflections	18745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	pH 7.0, VAPOR DIFFUSION, temperature 277K