2YXB

Crystal structure of the methylmalonyl-CoA mutase alpha-subunit from Aeropyrum pernix

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-15
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97884, 0.90000, 0.97938
Spacegroup name	P 21 21 21
Unit cell lengths	42.902, 51.134, 54.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.270 - 1.800
R-factor	0.21373
Rwork	0.212
R-free	0.24713
Structure solution method	MAD
RMSD bond length	0.033
RMSD bond angle	2.166
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	11582
<I/σ(I)>	17.8	2.6
Completeness [%]	99.8	98.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	oil batch		293	0.4M Ammonium dihydrogen phosphate, oil batch, temperature 293K